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Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs) but it has been difficult to p...
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| Vydáno v: | PLoS One |
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| Hlavní autoři: | , , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2016
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5082856/ https://ncbi.nlm.nih.gov/pubmed/27788226 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0165473 |
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