Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas

Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs) but it has been difficult to p...

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Publicado en:PLoS One
Autores principales: Kuniyoshi, Yuki, Maehara, Kazumitsu, Iwasaki, Takeshi, Hayashi, Masayasu, Semba, Yuichiro, Fujita, Masatoshi, Sato, Yuko, Kimura, Hiroshi, Harada, Akihito, Ohkawa, Yasuyuki
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5082856/
https://ncbi.nlm.nih.gov/pubmed/27788226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0165473
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