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Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

To analyze the immunoglobulin repertoire of human IgM+ B cells and the CD5(+) and CD5(-) subsets, individual CD19(+)/ IgM+/CD5(+) or CD5(-) B cells were sorted and non-productive as well as productive VH gene rearrangements were amplified from genomic DNA and sequenced. In both subsets, the VH3 fami...

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Autors principals: Brezinschek, H P, Foster, S J, Brezinschek, R I, Dörner, T, Domiati-Saad, R, Lipsky, P E
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC508090/
https://ncbi.nlm.nih.gov/pubmed/9153293
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