Genetic disease detection and DNA amplification using cloned thermostable ligase.
Polymerase chain reaction, using thermostable DNA polymerase, has revolutionized DNA diagnostics. Another thermostable enzyme, DNA ligase, is harnessed in the assay reported here that both amplifies DNA and discriminates a single-base substitution. This cloned enzyme specifically links two adjacent...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50775/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1986365/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.1.189 |
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