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Coupling capillary zone electrophoresis to a Q Exactive HF mass spectrometer for top-down proteomics: 580 proteoform identifications from yeast

We used reversed-phase liquid chromatography to separate the yeast proteome into 23 fractions. These fractions were then analyzed using capillary zone electrophoresis (CZE) coupled to a Q-Exactive HF mass spectrometer using an electrokinetically pumped sheath flow interface. The parameters of the ma...

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Detalhes bibliográficos
Publicado no:J Proteome Res
Main Authors: Zhao, Yimeng, Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5075424/
https://ncbi.nlm.nih.gov/pubmed/27490796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.6b00493
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