A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis

Cell-free protein synthesis (CFPS) systems are important laboratory tools that are used for various synthetic biology applications. Here, we present a simple and inexpensive laboratory-scale method for preparing a CFPS system from E. coli. The procedure uses basic lab equipment, a minimal set of rea...

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Enregistré dans:
Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Krinsky, Nitzan, Kaduri, Maya, Shainsky-Roitman, Janna, Goldfeder, Mor, Ivanir, Eran, Benhar, Itai, Shoham, Yuval, Schroeder, Avi
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5074584/
https://ncbi.nlm.nih.gov/pubmed/27768741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0165137
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