A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis

Cell-free protein synthesis (CFPS) systems are important laboratory tools that are used for various synthetic biology applications. Here, we present a simple and inexpensive laboratory-scale method for preparing a CFPS system from E. coli. The procedure uses basic lab equipment, a minimal set of rea...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Krinsky, Nitzan, Kaduri, Maya, Shainsky-Roitman, Janna, Goldfeder, Mor, Ivanir, Eran, Benhar, Itai, Shoham, Yuval, Schroeder, Avi
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5074584/
https://ncbi.nlm.nih.gov/pubmed/27768741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0165137
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