Quantification of DNA Extracted from Formalin Fixed Paraffin-Embeded Tissue Comparison of Three Techniques: Effect on PCR Efficiency

INTRODUCTION: Mutation detection from Formalin Fixed Paraffin-Embedding (FFPE) tissue in molecular lab became a necessary tool for defining potential targeted drug. Accurate quantification of DNA extracted from FFPE tissue is necessary for downstream applications like Polymerase Chain Reaction (PCR)...

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Udgivet i:J Clin Diagn Res
Main Authors: Kumar, Dushyant, Panigrahi, Manoj Kumar, Suryavanshi, Moushumi, Mehta, Anurag, Saikia, Kandarpa Kumar
Format: Artigo
Sprog:Inglês
Udgivet: JCDR Research and Publications (P) Limited 2016
Fag:
Biotechnology Section
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5071919/
https://ncbi.nlm.nih.gov/pubmed/27790419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7860/JCDR/2016/19383.8407
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