Application of RT-PCR in formalin-fixed and paraffin-embedded lung cancer tissues

AIM: To analyze gene expression in formalin-fixed, paraffin-embedded lung cancer tissues using modified method. METHODS: Total RNA from frozen tissues was extracted using TRIZOL reagent. RNA was extracted from formalin-fixed, paraffin-embedded tissues by digestion with proteinase K before the acid-p...

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Detalhes bibliográficos
Main Authors: Zhang, Fan, Wang, Zhuo-min, Liu, Hong-yu, Bai, Yun, Wei, Sen, Li, Ying, Wang, Min, Chen, Jun, Zhou, Qing-hua
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2010
Assuntos:
Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4002694/
https://ncbi.nlm.nih.gov/pubmed/20037605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/aps.2009.178
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