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Identification of amino acids related to catalytic function of Sulfolobus solfataricus P1 carboxylesterase by site-directed mutagenesis and molecular modeling

The archaeon Sulfolobus solfataricus P1 carboxylesterase is a thermostable enzyme with a molecular mass of 33.5 kDa belonging to the mammalian hormone-sensitive lipase (HSL) family. In our previous study, we purified the enzyme and suggested the expected amino acids related to its catalysis by chemi...

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Detalhes bibliográficos
Publicado no:BMB Rep
Main Authors: Choi, Yun-Ho, Lee, Ye-Na, Park, Young-Jun, Yoon, Sung-Jin, Lee, Hee-Bong
Formato: Artigo
Idioma:Inglês
Publicado em: Korean Society for Biochemistry and Molecular Biology 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5070724/
https://ncbi.nlm.nih.gov/pubmed/27222124
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5483/BMBRep.2016.49.6.077
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