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Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system
Despite many methodological advances that have facilitated investigation of Mycobacterium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen remains difficult. Here, we describe an optimized CRISPR-based method to inhibit expression of essential genes based...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5062980/ https://ncbi.nlm.nih.gov/pubmed/27407107 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw625 |
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