Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli

Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (His)(6) tags at the C-terminus (Chit1-V5-His) in the...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Kimura, Masahiro, Wakita, Satoshi, Ishikawa, Kotarou, Sekine, Kazutaka, Yoshikawa, Satoshi, Sato, Akira, Okawa, Kazuaki, Kashimura, Akinori, Sakaguchi, Masayoshi, Sugahara, Yasusato, Yamanaka, Daisuke, Ohno, Naohito, Bauer, Peter O, Oyama, Fumitaka
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5055312/
https://ncbi.nlm.nih.gov/pubmed/27716783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0164367
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