Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli

Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (His)(6) tags at the C-terminus (Chit1-V5-His) in the...

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發表在:PLoS One
Main Authors: Kimura, Masahiro, Wakita, Satoshi, Ishikawa, Kotarou, Sekine, Kazutaka, Yoshikawa, Satoshi, Sato, Akira, Okawa, Kazuaki, Kashimura, Akinori, Sakaguchi, Masayoshi, Sugahara, Yasusato, Yamanaka, Daisuke, Ohno, Naohito, Bauer, Peter O, Oyama, Fumitaka
格式: Artigo
語言:Inglês
出版: Public Library of Science 2016
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5055312/
https://ncbi.nlm.nih.gov/pubmed/27716783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0164367
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