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Sequencing of first-strand cDNA library reveals full-length transcriptomes

Massively parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling complex transcriptomes. However, many current methods for ssRNA-seq suffer from the underrepresentation of both the 5′ and 3′ ends of RNAs, which can be attributed to second-strand cDNA synt...

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Foilsithe in:Nat Commun
Main Authors: Agarwal, Saurabh, Macfarlan, Todd S., Sartor, Maureen A., Iwase, Shigeki
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5054741/
https://ncbi.nlm.nih.gov/pubmed/25607527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms7002
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