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Sequencing of first-strand cDNA library reveals full-length transcriptomes
Massively parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling complex transcriptomes. However, many current methods for ssRNA-seq suffer from the underrepresentation of both the 5′ and 3′ ends of RNAs, which can be attributed to second-strand cDNA synt...
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| Udgivet i: | Nat Commun |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5054741/ https://ncbi.nlm.nih.gov/pubmed/25607527 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms7002 |
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