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A Multifunctional Lentiviral-Based Gene Knockdown with Concurrent Rescue that Controls for Off-Target Effects of RNAi

The efficient, stable delivery of siRNA into cells, and the appropriate controls for non-specific off-target effects of siRNA are major limitations to functional studies using siRNA technology. To overcome these drawbacks, we have developed a single lentiviral vector that can concurrently deplete en...

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Detalles Bibliográficos
Publicado en:Genomics Proteomics Bioinformatics
Main Authors: Feng, Yunfeng, Nie, Linghu, Thakur, Meghna Das, Su, Qin, Chi, Zhenfen, Zhao, Yongliang, Longmore, Gregory D.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5054148/
https://ncbi.nlm.nih.gov/pubmed/21382592
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S1672-0229(10)60025-3
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