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Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium

Whole-genome sequencing is a useful approach for identification of chemical-induced lesions, but previous applications involved tedious genetic mapping to pinpoint the causative mutations. We propose that saturation mutagenesis under low mutagenic loads, followed by whole-genome sequencing, should a...

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Bibliografske podrobnosti
izdano v:Genome Res
Main Authors: Li, Cheng-Lin Frank, Santhanam, Balaji, Webb, Amanda Nicole, Zupan, Blaž, Shaulsky, Gad
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5052037/
https://ncbi.nlm.nih.gov/pubmed/27307293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.205682.116
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