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A vector platform for the rapid and efficient engineering of stable complex transgenes
We describe the generation of a set of plasmid vector tools that allow the rapid generation of complex-interacting stable transgenes in immortalized and primary cells. Of particular importance is inclusion of a mechanism to monitor the activation status of regulatory pathways via a reporter cassette...
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Publicado no: | Sci Rep |
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Main Authors: | , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Nature Publishing Group
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5046065/ https://ncbi.nlm.nih.gov/pubmed/27694838 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep34365 |
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