Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

High-throughput sequencing libraries are typically limited by the requirement for nanograms to micrograms of input DNA. This bottleneck impedes the microscale analysis of ecosystems and the exploration of low biomass samples. Current methods for amplifying environmental DNA to bypass this bottleneck...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:PeerJ
Autori principali: Rinke, Christian, Low, Serene, Woodcroft, Ben J., Raina, Jean-Baptiste, Skarshewski, Adam, Le, Xuyen H., Butler, Margaret K., Stocker, Roman, Seymour, Justin, Tyson, Gene W., Hugenholtz, Philip
Natura: Artigo
Lingua:Inglês
Pubblicazione: PeerJ Inc. 2016
Soggetti:
Genomics
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5036114/
https://ncbi.nlm.nih.gov/pubmed/27688978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.2486
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