Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

High-throughput sequencing libraries are typically limited by the requirement for nanograms to micrograms of input DNA. This bottleneck impedes the microscale analysis of ecosystems and the exploration of low biomass samples. Current methods for amplifying environmental DNA to bypass this bottleneck...

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Detalhes bibliográficos
Publicado no:PeerJ
Main Authors: Rinke, Christian, Low, Serene, Woodcroft, Ben J., Raina, Jean-Baptiste, Skarshewski, Adam, Le, Xuyen H., Butler, Margaret K., Stocker, Roman, Seymour, Justin, Tyson, Gene W., Hugenholtz, Philip
Formato: Artigo
Idioma:Inglês
Publicado em: PeerJ Inc. 2016
Assuntos:
Genomics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5036114/
https://ncbi.nlm.nih.gov/pubmed/27688978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.2486
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