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Topography of Cells Revealed by Variable-Angle Total Internal Reflection Fluorescence Microscopy

We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theo...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Cardoso Dos Santos, Marcelina, Déturche, Régis, Vézy, Cyrille, Jaffiol, Rodolphe
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5034310/
https://ncbi.nlm.nih.gov/pubmed/27653490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2016.06.043
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