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A Targeted Mutation Identified through pK(a) Measurements Indicates a Postrecruitment Role for Fis1 in Yeast Mitochondrial Fission
The tail-anchored protein Fis1 is implicated as a passive tether in yeast mitochondrial fission. We probed the functional role of Fis1 Glu-78, whose elevated side chain pK(a) suggests participation in protein interactions. Fis1 binds partners Mdv1 or Dnm1 tightly, but mutation E78A weakens Fis1 inte...
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| Udgivet i: | J Biol Chem |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Biochemistry and Molecular Biology
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5034033/ https://ncbi.nlm.nih.gov/pubmed/27496949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.724005 |
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