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The Kinesin-5 Chemomechanical Cycle Is Dominated by a Two-heads-bound State
Single-molecule microscopy and stopped-flow kinetics assays were carried out to understand the microtubule polymerase activity of kinesin-5 (Eg5). Four lines of evidence argue that the motor primarily resides in a two-heads-bound (2HB) state. First, upon microtubule binding, dimeric Eg5 releases bot...
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| Publicat a: | J Biol Chem |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Biochemistry and Molecular Biology
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5034029/ https://ncbi.nlm.nih.gov/pubmed/27402829 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.730697 |
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