Identification of Specific Effect of Chloride on the Spectral Properties and Structural Stability of Multiple Extracellular Glutamic Acid Mutants of Bacteriorhodopsin

In the present work we combine spectroscopic, DSC and computational approaches to examine the multiple extracellular Glu mutants E204Q/E194Q, E204Q/E194Q/E9Q and E204Q/E194Q/E9Q/E74Q of bacteriorhodopsin by varying solvent ionic strength and composition. Absorption spectroscopy data reveal that the...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Lazarova, Tzvetana, Mlynarczyk, Krzysztof, Querol, Enric, Tenchov, Boris, Filipek, Slawomir, Padrós, Esteve
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5033488/
https://ncbi.nlm.nih.gov/pubmed/27657718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0162952
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