Identification of Specific Effect of Chloride on the Spectral Properties and Structural Stability of Multiple Extracellular Glutamic Acid Mutants of Bacteriorhodopsin

In the present work we combine spectroscopic, DSC and computational approaches to examine the multiple extracellular Glu mutants E204Q/E194Q, E204Q/E194Q/E9Q and E204Q/E194Q/E9Q/E74Q of bacteriorhodopsin by varying solvent ionic strength and composition. Absorption spectroscopy data reveal that the...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Lazarova, Tzvetana, Mlynarczyk, Krzysztof, Querol, Enric, Tenchov, Boris, Filipek, Slawomir, Padrós, Esteve
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5033488/
https://ncbi.nlm.nih.gov/pubmed/27657718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0162952
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