A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells

While Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines, the CRISPR-Cas9 system can introduce undesirable “on-target” mutations within the second allele of successfully modified cells via non-homologous end joining (NHEJ). To address this, we fused the Streptococcus pyog...

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Bibliographic Details
Published in:Stem Cell Reports
Main Authors: Howden, Sara E., McColl, Bradley, Glaser, Astrid, Vadolas, Jim, Petrou, Steven, Little, Melissa H., Elefanty, Andrew G., Stanley, Edouard G.
Format: Artigo
Language:Inglês
Published: Elsevier 2016
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5031952/
https://ncbi.nlm.nih.gov/pubmed/27499201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2016.07.001
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