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Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells

CRISPR/Cas9 nucleases have enabled powerful, new genome editing capabilities; however, the preponderance of non-homologous end joining (NHEJ) mediated repair events over homology directed repair (HDR) in most cell types limits the ability to engineer precise changes in mammalian genomes. Here, we in...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Shy, Brian R., MacDougall, Matthew S., Clarke, Ryan, Merrill, Bradley J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5027516/
https://ncbi.nlm.nih.gov/pubmed/27484482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw685
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