Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli

Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria. Two systems widely used in Escherichia coli are the Red system from phage λ and RecET from the defective Rac prophage. We investigate...

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Bibliographic Details
Published in:mBio
Main Authors: Thomason, Lynn C., Costantino, Nina, Court, Donald L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5021808/
https://ncbi.nlm.nih.gov/pubmed/27624131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01443-16
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