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Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides

The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. While the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory circuitry of nucleotide exchange executed by oligonucleotides alo...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Bialk, Pawel, Sansbury, Brett, Rivera-Torres, Natalia, Bloh, Kevin, Man, Dula, Kmiec, Eric B.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5016854/
https://ncbi.nlm.nih.gov/pubmed/27609304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep32681
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