Demonstration of mRNA using digoxigenin labelled oligonucleotide probes for in situ hybridisation in formamide free conditions.

The value of formamide for use in in situ hybridisation (ISH) for the detection of mRNA, using either single or multi probe cocktails of digoxigenin labelled oligoprobes, was investigated. Three peptides with cell specific localisation in three separate tissues--calcitonin in the thyroid, epidermal...

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Detalhes bibliográficos

Publicado no:	J Clin Pathol
Principais autores:	Thomas, G A, Davies, H G, Williams, E D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMJ Publishing Group 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC501152/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8459039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.46.2.171
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