Striking effects of coupling mutations in the acceptor stem on recognition of tRNAs by Escherichia coli Met-tRNA synthetase and Met-tRNA transformylase.

We measured kinetic parameters in vitro and directly analyzed aminoacylation and formylation levels in vivo to study recognition of Escherichia coli initiator tRNA mutants by E. coli Met-tRNA synthetase and Met-tRNA transformylase. We show that, in addition to the anticodon sequence, mutations in th...

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Библиографические подробности

Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Lee, C P, Dyson, M R, Mandal, N, Varshney, U, Bahramian, B, RajBhandary, U L
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 1992
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50106/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409632/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.19.9262
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