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Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction.

AIM: To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). METHODS: The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T g...

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Bibliografski detalji
Izdano u:J Clin Pathol
Glavni autori: Lee, P Y, Mangan, J, Holliman, R E, Butcher, P D
Format: Artigo
Jezik:Inglês
Izdano: BMJ Publishing Group 1999
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC501009/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10343614/
https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.52.1.61
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