Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction.

AIM: To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). METHODS: The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T g...

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Detalles Bibliográficos
Autores principales: Lee, P Y, Mangan, J, Holliman, R E, Butcher, P D
Formato: Artigo
Lenguaje:Inglês
Publicado: 1999
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC501009/
https://ncbi.nlm.nih.gov/pubmed/10343614
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