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Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line

CRISPR/Cas9 system has been used widely in animals and plants to direct mutagenesis. To date, no such method exists for fish somatic cell lines. We describe an efficient procedure for genome editing in the Chinook salmon Oncorhynchus tshawytscha CHSE. This cell line was genetically modified to first...

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Detalhes bibliográficos
Publicado no:Mar Biotechnol (NY)
Main Authors: Dehler, Carola E., Boudinot, Pierre, Martin, Samuel A. M., Collet, Bertrand
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5007268/
https://ncbi.nlm.nih.gov/pubmed/27236514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10126-016-9708-6
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