Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antis...

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Bibliografiske detaljer
Udgivet i:Biol Open
Main Authors: Nakagawa, Yoshiko, Sakuma, Tetsushi, Nishimichi, Norihisa, Yokosaki, Yasuyuki, Yanaka, Noriyuki, Takeo, Toru, Nakagata, Naomi, Yamamoto, Takashi
Format: Artigo
Sprog:Inglês
Udgivet: The Company of Biologists Ltd 2016
Fag:
Methods & Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5004614/
https://ncbi.nlm.nih.gov/pubmed/27387532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.019349
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