A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation.

AIM: To determine the optimum form of labelling and the most efficient reporter molecule for non-radioisotopic in situ hybridisation (ISH). METHODS: Nine deoxyoligonucleotides complementary to histone mRNA were synthesised and labelled either enzymatically or during solid-phase synthesis with the re...

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Detalhes bibliográficos

Publicado no:	J Clin Pathol
Principais autores:	Harper, S J, Bailey, E, McKeen, C M, Stewart, A S, Pringle, J H, Feehally, J, Brown, T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMJ Publishing Group 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC500121/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9301555/ https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.50.8.686
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