High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.
This report describes a method for growing both bloodstream- and procyclic-form Trypanosoma brucei as colonies on agarose plates. Procyclic colonies, which took 2 weeks to develop, grew with approximately 17% plating efficiency on SDM-79/0.65% agarose supplemented with 20% (vol/vol) conditioned medi...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50012/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1528898/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.18.8818 |
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