High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.
This report describes a method for growing both bloodstream- and procyclic-form Trypanosoma brucei as colonies on agarose plates. Procyclic colonies, which took 2 weeks to develop, grew with approximately 17% plating efficiency on SDM-79/0.65% agarose supplemented with 20% (vol/vol) conditioned medi...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50012/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1528898/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.18.8818 |
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