Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H(+) Ions and Warrants Further Studies of Organellar pH

Fluorescence measurements of pH and other analytes in the cell rely on accurate calibrations, but these have routinely used algorithms that inadequately describe the properties of indicators. Here, we have established a more accurate method for calibrating and analyzing data obtained using the ratio...

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Publicat a:PLoS One
Autors principals: Żurawik, Tomasz Michał, Pomorski, Adam, Belczyk-Ciesielska, Agnieszka, Goch, Grażyna, Niedźwiedzka, Katarzyna, Kucharczyk, Róża, Krężel, Artur, Bal, Wojciech
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2016
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4996429/
https://ncbi.nlm.nih.gov/pubmed/27557123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0161353
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