Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of multiple co-existing complexes. Sedimentation v...

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Veröffentlicht in:eLife
Hauptverfasser: Zhao, Huaying, Fu, Yan, Glasser, Carla, Andrade Alba, Eric J, Mayer, Mark L, Patterson, George, Schuck, Peter
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2016
Schlagworte:
Biophysics and Structural Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4985284/
https://ncbi.nlm.nih.gov/pubmed/27436096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.17812
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