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High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize
BACKGROUND: CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice. RESULTS: We optimized and introduced the multiplex gene editing strategy ba...
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| Publicado no: | BMC Biotechnol |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4982333/ https://ncbi.nlm.nih.gov/pubmed/27515683 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0289-2 |
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