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Distinct oxidative cleavage and modification of bovine [Cu-Zn]-SOD by an ascorbic acid/Cu(II) system: Identification of novel copper binding site on SOD molecule

We investigated the combined effect of ascorbate and copper [Asc/Cu(II)] on the integrity of bovine [Cu-Zn]-superoxide dismutase (bSOD1) as a model system to study the metal catalyzed oxidation (MCO) and fragmentation of proteins. We found Asc/Cu(II) mediates specific cleavage of bSOD1 and generates...

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Detalhes bibliográficos
Publicado no:Free Radic Biol Med
Main Authors: Uehara, Hiroshi, Luo, Shen, Aryal, Baikuntha, Levine, Rodney L., Rao, V. Ashutosh
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4979071/
https://ncbi.nlm.nih.gov/pubmed/26872685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2016.01.020
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