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A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells

We report here a method for the use of poly-l-lysine (PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells (BMSCs) in culture and in situ, which are typically difficult to transduce. We show by fluorescence microscopy and flow cy...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Bone Res
Hauptverfasser: Buo, Atum M, Williams, Mark S, Kerr, Jaclyn P, Stains, Joseph P
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4977485/
https://ncbi.nlm.nih.gov/pubmed/27547486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/boneres.2016.21
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