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A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells

We report here a method for the use of poly-l-lysine (PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells (BMSCs) in culture and in situ, which are typically difficult to transduce. We show by fluorescence microscopy and flow cy...

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Detalhes bibliográficos
Publicado no:Bone Res
Main Authors: Buo, Atum M, Williams, Mark S, Kerr, Jaclyn P, Stains, Joseph P
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4977485/
https://ncbi.nlm.nih.gov/pubmed/27547486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/boneres.2016.21
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