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A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells

We report here a method for the use of poly-l-lysine (PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells (BMSCs) in culture and in situ, which are typically difficult to transduce. We show by fluorescence microscopy and flow cy...

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Publicat a:Bone Res
Autors principals: Buo, Atum M, Williams, Mark S, Kerr, Jaclyn P, Stains, Joseph P
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2016
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4977485/
https://ncbi.nlm.nih.gov/pubmed/27547486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/boneres.2016.21
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