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A simple but precise method for quantitative measurement of the quality of the laser focus in a scanning optical microscope
We report a method for characterizing the focussing laser beam exiting the objective in a laser scanning microscope. This method provides the size of the optical focus, the divergence of the beam, the ellipticity and the astigmatism. We use a microscopic‐scale knife edge in the form of a simple tran...
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| Veröffentlicht in: | J Microsc |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4975716/ https://ncbi.nlm.nih.gov/pubmed/25864964 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jmi.12249 |
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