Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction

BACKGROUND: Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromo...

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Detalhes bibliográficos
Publicado no:BMC Cell Biol
Main Authors: Codner, Gemma F., Lindner, Loic, Caulder, Adam, Wattenhofer-Donzé, Marie, Radage, Adam, Mertz, Annelyse, Eisenmann, Benjamin, Mianné, Joffrey, Evans, Edward P., Beechey, Colin V., Fray, Martin D., Birling, Marie-Christine, Hérault, Yann, Pavlovic, Guillaume, Teboul, Lydia
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4974727/
https://ncbi.nlm.nih.gov/pubmed/27496052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12860-016-0108-6
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