Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction

BACKGROUND: Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromo...

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Publicat a:BMC Cell Biol
Autors principals: Codner, Gemma F., Lindner, Loic, Caulder, Adam, Wattenhofer-Donzé, Marie, Radage, Adam, Mertz, Annelyse, Eisenmann, Benjamin, Mianné, Joffrey, Evans, Edward P., Beechey, Colin V., Fray, Martin D., Birling, Marie-Christine, Hérault, Yann, Pavlovic, Guillaume, Teboul, Lydia
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Methodology Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4974727/
https://ncbi.nlm.nih.gov/pubmed/27496052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12860-016-0108-6
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