Replication-Competent Influenza A Viruses Expressing Reporter Genes

Influenza A viruses (IAV) cause annual seasonal human respiratory disease epidemics. In addition, IAV have been implicated in occasional pandemics with inordinate health and economic consequences. Studying IAV, in vitro or in vivo, requires the use of laborious secondary methodologies to identify vi...

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Izdano u:Viruses
Glavni autori: Breen, Michael, Nogales, Aitor, Baker, Steven F., Martínez-Sobrido, Luis
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2016
Teme:
Review
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4974514/
https://ncbi.nlm.nih.gov/pubmed/27347991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v8070179
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spelling pubmed-49745142016-08-08 Replication-Competent Influenza A Viruses Expressing Reporter Genes Breen, Michael Nogales, Aitor Baker, Steven F. Martínez-Sobrido, Luis Viruses Review Influenza A viruses (IAV) cause annual seasonal human respiratory disease epidemics. In addition, IAV have been implicated in occasional pandemics with inordinate health and economic consequences. Studying IAV, in vitro or in vivo, requires the use of laborious secondary methodologies to identify virus-infected cells. To circumvent this requirement, replication-competent IAV expressing an easily traceable reporter protein can be used. Here we discuss the development and applications of recombinant replication-competent IAV harboring diverse fluorescent or bioluminescent reporter genes in different locations of the viral genome. These viruses have been employed for in vitro and in vivo studies, such as the screening of neutralizing antibodies or antiviral compounds, the identification of host factors involved in viral replication, cell tropism, the development of vaccines, or the assessment of viral infection dynamics. In summary, reporter-expressing, replicating-competent IAV represent a powerful tool for the study of IAV both in vitro and in vivo. MDPI 2016-06-23 /pmc/articles/PMC4974514/ /pubmed/27347991 http://dx.doi.org/10.3390/v8070179 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Review
spellingShingle Review
Breen, Michael
Nogales, Aitor
Baker, Steven F.
Martínez-Sobrido, Luis
Replication-Competent Influenza A Viruses Expressing Reporter Genes
description Influenza A viruses (IAV) cause annual seasonal human respiratory disease epidemics. In addition, IAV have been implicated in occasional pandemics with inordinate health and economic consequences. Studying IAV, in vitro or in vivo, requires the use of laborious secondary methodologies to identify virus-infected cells. To circumvent this requirement, replication-competent IAV expressing an easily traceable reporter protein can be used. Here we discuss the development and applications of recombinant replication-competent IAV harboring diverse fluorescent or bioluminescent reporter genes in different locations of the viral genome. These viruses have been employed for in vitro and in vivo studies, such as the screening of neutralizing antibodies or antiviral compounds, the identification of host factors involved in viral replication, cell tropism, the development of vaccines, or the assessment of viral infection dynamics. In summary, reporter-expressing, replicating-competent IAV represent a powerful tool for the study of IAV both in vitro and in vivo.
author Breen, Michael
Nogales, Aitor
Baker, Steven F.
Martínez-Sobrido, Luis
author_facet Breen, Michael
Nogales, Aitor
Baker, Steven F.
Martínez-Sobrido, Luis
author_sort Breen, Michael
title Replication-Competent Influenza A Viruses Expressing Reporter Genes
title_short Replication-Competent Influenza A Viruses Expressing Reporter Genes
title_full Replication-Competent Influenza A Viruses Expressing Reporter Genes
title_fullStr Replication-Competent Influenza A Viruses Expressing Reporter Genes
title_full_unstemmed Replication-Competent Influenza A Viruses Expressing Reporter Genes
title_sort replication-competent influenza a viruses expressing reporter genes
publisher MDPI
container_title Viruses
publishDate 2016
url https://ncbi.nlm.nih.gov/pmc/articles/PMC4974514/
https://ncbi.nlm.nih.gov/pubmed/27347991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v8070179
_version_ 1816666740380139520

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