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Refolding and oriented insertion of a membrane protein into a lipid bilayer.

We have studied the refolding and membrane insertion of the outer membrane protein OmpA of Escherichia coli. The protein was extracted from its native membrane by sonication in the presence of urea and dissolved in the urea/water mixture in unfolded form. In this form it was purified. Upon addition...

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Bibliografski detalji
Izdano u:Proc Natl Acad Sci U S A
Glavni autori: Surrey, T, Jähnig, F
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1992
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49729/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1502158/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.16.7457
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