Refolding and oriented insertion of a membrane protein into a lipid bilayer.
We have studied the refolding and membrane insertion of the outer membrane protein OmpA of Escherichia coli. The protein was extracted from its native membrane by sonication in the presence of urea and dissolved in the urea/water mixture in unfolded form. In this form it was purified. Upon addition...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1992
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49729/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1502158/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.16.7457 |
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