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Protocol 1: Verifying the function and localization of genetically-encoded Ca(2+) sensors and converting FRET ratios to Ca(2+) concentrations
Genetically-encoded, ratiometric, fluorescent, Ca(2+) biosensors can be used in living cells to quantitatively measure free Ca(2+) concentrations in the cytosol or in organelles. This protocol describes how to perform a calibration of a Ca(2+) sensor expressed in cultured mammalian cells as images a...
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| Publicado en: | Cold Spring Harb Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4968932/ https://ncbi.nlm.nih.gov/pubmed/25561614 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot076547 |
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