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Quantitative measurement of Ca(2+) and Zn(2+) in mammalian cells using genetically encoded fluorescent biosensors

Genetically encoded, ratiometric, fluorescent biosensors can be used to quantitatively measure intracellular ion concentrations in living cells. We describe important factors to consider when selecting a Ca(2+) or Zn(2+) biosensor, such as the sensor’s dissociation constant (K(d)’) and its dynamic r...

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Detalhes bibliográficos
Main Authors: Park, J. Genevieve, Palmer, Amy E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4051312/
https://ncbi.nlm.nih.gov/pubmed/24052378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-622-1_3
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