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Quantitative measurement of Ca(2+) and Zn(2+) in mammalian cells using genetically encoded fluorescent biosensors
Genetically encoded, ratiometric, fluorescent biosensors can be used to quantitatively measure intracellular ion concentrations in living cells. We describe important factors to consider when selecting a Ca(2+) or Zn(2+) biosensor, such as the sensor’s dissociation constant (K(d)’) and its dynamic r...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4051312/ https://ncbi.nlm.nih.gov/pubmed/24052378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-622-1_3 |
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